We study the effect of primordial isocurvature perturbations on non-Gaussian properties of CMB temperature anisotropies . We consider generic forms of the non-linearity of isocurvature perturbations which can be applied to a wide range of theoretical models . We derive analytical expressions for the bispectrum and the Minkowski Functionals for CMB temperature fluctuations to describe the non-Gaussianity from isocurvature perturbations . We find that the isocurvature non-Gaussianity in the quadratic isocurvature model , where the isocurvature perturbation S is written as a quadratic function of the Gaussian variable \sigma , S = \sigma ^ { 2 } - \langle \sigma ^ { 2 } \rangle , can give the same signal-to-noise as f _ { NL } = 30 even if we impose the current observational limit on the fraction of isocurvature perturbations contained in the primordial power spectrum \alpha . We give constraints on isocurvature non-Gaussianity from Minkowski Functionals using the WMAP 5-year data . We do not find a significant signal of isocurvature non-Gaussianity . For the quadratic isocurvature model , we obtain a stringent upper limit on the isocurvature fraction \alpha < 0.070 ( 95 % CL ) for a scale invariant spectrum which is comparable to the limit obtained from the power spectrum .