We present a comprehensive study of the total X-ray emission from the colliding galaxy pair NGC 2207/IC 2163 , based on Chandra , Spitzer , and GALEX data . We detect 28 ultra-luminous X-ray sources ( ‘ ULXs ’ ) , 7 of which were not detected previously due to X-ray variability . Twelve sources show significant long-term variability , with no correlated spectral changes . Seven sources are transient candidates . One ULX coincides with an extremely blue star cluster ( B-V = -0.7 ) . We confirm that the global relation between the number and luminosity of ULXs and the integrated star formation rate ( ‘ SFR ’ ) of the host galaxy also holds on local scales . We investigate the effects of dust extinction and/or age on the X-ray binary ( ‘ XRB ’ ) population on sub-galactic scales . The distributions of N _ { X } and L _ { X } are peaked at L _ { IR } / L _ { NUV } \sim 1 , which may be associated with an age of \sim 10 Myr for the underlying stellar population . We find that \sim 1 / 3 of the XRBs are located in close proximity to young star complexes . The luminosity function of the X-ray binaries ( ‘ XRBs ’ ) is consistent with that typical for high-mass X-ray binaries , and appears unaffected by variability . We disentangle and compare the X-ray diffuse spectrum with that of the bright XRBs . The hot interstellar medium dominates the diffuse X-ray emission at E \lesssim 1 keV , has a temperature kT = 0.28 ^ { +0.05 } _ { -0.04 } keV and intrinsic 0.5-2 keV luminosity of 7.9 \times 10 ^ { 40 } erg s ^ { -1 } , a factor of \sim 2.3 higher than the average thermal luminosity produced per unit SFR in local star-forming galaxies . The total X-ray output of NGC 2207/IC 2163 is 1.5 \times 10 ^ { 41 } ergs s ^ { -1 } , and the corresponding total integrated SFR is 23.7 M _ { \odot } yr ^ { -1 } .