Context : Aims : This work presents an extensive study of the previously discovered formation of bipolar flux concentrations in a two-layer model . We interpret the formation process in terms of negative effective magnetic pressure instability ( NEMPI ) , which is a possible mechanism to explain the origin of sunspots . Methods : In our simulations , we use a Cartesian domain of isothermal stratified gas that is divided into two layers . In the lower layer , turbulence is forced with transverse nonhelical random waves , whereas in the upper layer no flow is induced . A weak uniform magnetic field is imposed in the entire domain at all times . In most cases , it is horizontal , but a vertical and an inclined field are also considered . In this study we vary the stratification by changing the gravitational acceleration , magnetic Reynolds number , strength of the imposed magnetic field , and size of the domain to investigate their influence on the formation process . Results : Bipolar magnetic structure formation takes place over a large range of parameters . The magnetic structures become more intense for higher stratification until the density contrast becomes around 100 across the turbulent layer . For the fluid Reynolds numbers considered , magnetic flux concentrations are generated at magnetic Prandtl number between 0.1 and 1 . The magnetic field in bipolar regions increases with higher imposed field strength until the field becomes comparable to the equipartition field strength of the turbulence . A larger horizontal extent enables the flux concentrations to become stronger and more coherent . The size of the bipolar structures turns out to be independent of the domain size . A small imposed horizontal field component is necessary to generate bipolar structures . In the case of bipolar region formation , we find an exponential growth of the large-scale magnetic field , which is indicative of a hydromagnetic instability . Additionally , the flux concentrations are correlated with strong large-scale downward and converging flows . These findings imply that NEMPI is responsible for magnetic flux concentrations . Conclusions :