We present the results from a wide-field spectroscopic survey of globular clusters ( GCs ) in the Virgo Cluster . We obtain spectra for 201 GCs and 55 ultracompact dwarfs ( UCDs ) using the Hectospec on the Multiple Mirror Telescope , and derive their radial velocities . We identify 46 genuine intracluster GCs ( IGCs ) , not associated with any Virgo galaxies , using the 3D GMM test on the spatial and radial velocity distribution . They are located at the projected distance 200 kpc \lesssim R \lesssim 500 kpc from the center of M87 . The radial velocity distribution of these IGCs shows two peaks , one at v _ { \mathrm { r } } = 1023 km s ^ { -1 } associated with the Virgo main body , and another at v _ { \mathrm { r } } = 36 km s ^ { -1 } associated with the infalling structure . The velocity dispersion of the IGCs in the Virgo main body is \sigma _ { GC } \sim 314 km s ^ { -1 } , which is smoothly connected to the velocity dispersion profile of M87 GCs , but much lower than that of dwarf galaxies in the same survey field , \sigma _ { dwarf } \sim 608 km s ^ { -1 } . The UCDs are more centrally concentrated on massive galaxies , M87 , M86 , and M84 . The radial velocity dispersion of the UCD system is much smaller than that of dwarf galaxies . Our results confirm the large-scale distribution of Virgo IGCs indicated by previous photometric surveys . The color distribution of the confirmed IGCs shows a bimodality similar to that of M87 GCs . This indicates that most IGCs are stripped off from dwarf galaxies and some from massive galaxies in the Virgo .