Results on timing and spectral properties of the Be/X-ray binary pulsar 3A 0726-260 ( 4U 0728-25 ) are presented . The binary was observed on 2016 May 6-7 with the Large Area X-ray Proportional Counter ( LAXPC ) and Soft X-ray Telescope ( SXT ) instruments onboard the AstroSat satellite . During this observation the source was in non-flaring persistent state at a flux level of \sim 8.6 \pm 0.3 \times 10 ^ { -11 } ergs cm ^ { -2 } sec ^ { -1 } in 0.4-20 keV . Strong X-ray pulsations with a period of 103.144 \pm 0.001 seconds are detected in 0.3-7 keV with the SXT and in 3-40 keV with the LAXPC . The pulse profile is energy dependent , and there is an indication that the pulse shape changes from a broad single pulse to a double pulse at higher energy . At energies above 20 keV , we report the first time detection of pulsation period 103.145 \pm 0.001 seconds and the double peaked pulse profile from the source . The energy spectrum of the source is derived from the combined analysis of the SXT and LAXPC spectral data in 0.4-20 keV . The best spectral fit is obtained by a power law model with a photon index ( 1.7 \pm 0.03 ) with high energy spectral cut-off at 12.9 \pm 0.7 keV . A broad Iron line at \sim 6.3 keV is detected in the energy spectrum . We briefly discuss the implications of these results .